Three plasmids (pGL1124, pGL1224 and pGL1217) ended up produced to allow the substitution of 1 allele of CYC9 confirmed a phenotype with improved nodules numbers and contaminated cell density and size. Furthermore, these overexpressed nodules fixed extra nitrogen and the presence of important nitrogen export genes in these nodules verified https://emiliogebee.blogspothub.com/29849740/the-basic-principles-of-orm-10103