POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to generate highly reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-rich moieties like tyrosine, phenylalanine and tryptophan at or close to the POD. The limited fifty percent-lifetime of the tyramide radicals restricts the deposition of tyramide3. https://popeo777alw0.wikienlightenment.com/user